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新能源材料与器件“实验班”系列报告之五十七:Developing genetic engineering platform for a filamentous cyanobacterium with wide range temperature tolerance
作者:材料学院  来源:材料学院  编辑:王文艳   点击:[] 日期:2019年06月10日

报告题目:Developing genetic engineering platform for a filamentous cyanobacterium with wide range temperature tolerance

报告地点:2019611日,下午14:00,四方校区一教207

报告人:Dr. Nitin Keshari, Ph.D.

报告人简历:

Dr. Nitin Keshari, Ph.D.

E-mail id: nitindna@gmail.com

Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China

Dr. Nitin Keshari completed his Masters degree from Post-Graduate Department of Biotechnology, Utkal University, India in 2010. He got his Ph.D. degree on the topic“Study of stress tolerant cyanobacteria colonizing stone monuments and characterization of their antioxidant defense systems”from Department of Biotechnology, Visva Bharati University, India in 2015. He worked as a Research Associate on“Algal flocculation and genetic modification for biofuel production”in International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India for one and half years. Presently he is working asPostdoctoral Fellow (Talented Young Scientist Program) on“Metabolic engineering and bioprospection of cyanobacteria”in Microbial Metabolic Engineering Group in Qingdao Institute of Bioenergy and Bioprocess Technology, China since 2017.He has experience of many training programmes/workshops on algae and he has also participated in many national and international conferences.

Cyanobacteria are a promising option as a cell factoryfor producinghigh-value bio-products dueto their ability to exploit sunlight and CO2as the sole energy and carbon sources respectively.Many cyanobacteria have been explored for production of valuable secondary metabolites and other products. The most common cyanobacterial model strains are unicellular cyanobacteriumSynechocystissp. plays a major role in the development of cyanobacterial biotechnology and very few multicellular/filamentous strains (AnabaenaPCC 7120,Leptolyngbyasp. etc.) are developed as model strains, but not robust. Our initial screening on the filamentous mat formingLeptolyngbyasp.(isolated from hot springs of Yunnan province) confirmed high and wide range of temperature tolerance (30-50°C) which opens an option to use this strain as commercial and model strain. As being thefilamentous cyanobacteria,Leptolyngbyastrains arebetter candidate than unicellular form because of its tendency to bio-flocculate or float to the surface of a pond for easier harvesting that advances cost effective production of bio-products at commercial scale.Genome engineering technologies enable the rational engineering and perturbation of cyanobacteria tobuild non-native and improved native biosynthetic pathways which displayed huge potential for biotechnology applications.Generating robust filamentous cyanobacteria as model system is less explored. In order to significantly improve the biotechnological implementation of stable and robust transgenic filamentous cyanobacteria, we propose to develop naturally isolated filamentous cyanobacteria as a new model system/cell factory to enhance the production of volatile compounds and other value-added products.

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